The experiments were done in triplicates. Orthofinder results of seven Poaceae genomes comparison. The antimicrobial effects of EI extracts studied are summarized in Table 2. M. S. thesis, Rutgers-the State University of New Jersey, 1959 and unpublished data. Five microliter of this solution was mixed with 100?l extract solutions in microtiter 96-well plates. https://doi Soil sample and seeds of E. inidca were collected from a farmland in Obe, Nkanu West L.G.A of Enugu State. Abstract and Figures Eleusine indica L. Gaertn also known as wire grass belonging to family poaceae. Available online at www.ijpab.com ISSN: 2320 – 7051 Int. have been detected in Asia, the Americas but not in Europe. Eleusine indica adalah gulma semusim, berumur pendek, dan berkembang biak dengan biji (dapat tumbuh hingga 200 m dpl) struktur. Ambayeba Muimba-Kankolongo, in Food Crop Production by Smallholder Farmers in Southern Africa, 2018. Plant extracts were initially dissolved in DMSO as mentioned earlier, with the final concentration of DMSO being 0.1% (v/v). Journal of Integrative Agriculture, Vol. A greenhouse experiment was performed to evaluate effectiveness of goosegrass (Eleusine indica) in phytoremediation of soil contaminated with 8,247 mg kg−1 of total petroleum hydrocarbons (TPH). In this respect, a part of our study was determining TPC in different extracts obtained from EI. Table S1. Wei Deng, Qian Yang, Yongrui Chen, Mengting Yang, Zhiming Xia, Jin Zhu, Yueyang Chen, Jingxuan Cai, Shuzhong Yuan, Cyhalofop-butyl and glyphosate multiple herbicide resistance evolved in an Eleusine indica population collected in Chinese direct-seeding rice, Journal of Agricultural and Food Chemistry, 10.1021/acs.jafc.9b07342, (2020). 14, Issue. However, R biotypes of goosegrass have arisen due … We will be providing unlimited waivers of publication charges for accepted research articles as well as case reports and case series related to COVID-19. Journal of Biodiversity and Environmental Sciences 13(4), 131-140. The TPC was expressed as gallic acid equivalents (GAE) in mg/g extract, obtained from the standard curve of gallic acid solutions. The ligule (a scale at the attachment of the leaf sheaf to the leaf blade) is membra-nous and fringed. The concentration which inhibited 50% of cellular growth (IC50 value) was determined and the inhibitory rate of cell proliferation was calculated by the following formula:
Goosegrass has a broad tolerance to a wide range of environmental conditions, but its vegetative growth is significantly reduced during dry seasons [2, 3]. Each plate included untreated cell controls and a blank cell-free control. 2016;29: 1875–1878. Review articles are excluded from this waiver policy. Pure App. Eleusine indica . Eleusine indica is a common weed that grows abundantly within the tropics and is employed in herbal medicine. Zainol, A. Abd-Hamid, S. Yusof, and R. Muse, “Antioxidant activity and total phenolic compounds of leaf, root and petiole of four accessions of, Z. Zhang, Q. Chang, M. Zhu, Y. Huang, W. K. K. Ho, and Z.-Y. Please check your email for instructions on resetting your password. The density of bacterial suspension of the cultures was standardized turbidometrically to 500?000–1?000?000 colony forming units per milliliter (CFU/ml) at wavelength of 600?nm. As a result, the MeTH of EI was showed to have the highest TPC when compared with DCM, hexane and EA extracts. The decoction possesses anti-s. strains. Rumput Belulang (Eleusine indica L.) merupakan salah satu gulma tanaman budidaya yang mempunyai daya saing yang tinggi dan keluarga Poaceae atau termasuk dalam keluarga rumput-rumputan. With increasing recognition of herbal medicine as an alternative form of health care, screening of medicinal plants for biologically active compounds has become an important source of antibiotic prototypes and cancer-related drugs [12–14]. To screen the extracts of EI, dried extracts of the plant were dissolved in 1?ml of DMSO to give a stock solution of extract at 10?mg/ml.
goosegrass. Conclusion:These results clearly show the antioxidant capacity and anti-inflammatory activities of plant extracts collected The aim of this study was to evaluate resistance levels and possible target site resistance mechanisms of an E. indica biotype (19-1) collected from a plant nursery in Southern Italy where poor glyphosate efficacy was reported. In addition, we also report 115,417 simple sequence repeats which can be deployed in population genetics and phylogenetic analysis. The antibacterial activities of the different extracts of EI were evaluated using both Gram-positive and Gram-negative bacteria. Plants produce a higher number of naturally occurring secondary metabolites, many of them with unique pharmacologic activities. 2623-2630 ISSN: 1520-5118 Eleusine indica, and Polyscias fulva. These metabolites include the flavonoids, phenols and phenolic glycosides, saponins, cyanogenic glycosides, unsaturated lactones and glucosinolates [5–8]. when compared with the MeTH.
DNH resistance in goosegrass [Eleusine indica (L.) Gaertn. Our assembly was able to identify all major herbicide‐resistance related genes and develop a useful tool for other genomic and evolutionary analysis. SHARMA Abstract E&wine Mica and Sporoboluspyrami are common grasses … Working off-campus? The use of evidence-based complementary and alternative medicine is increasing rapidly. – goose grass (Poaceae) (syn. Antibacterial and teratogenic activity of Eleusine indica leaf extracts. IOP PublishingUTILIZATION OF GOOSE GRASS (Eleusine indica) AND SAWDUST AS BIOMASS BRIQUETTES Journal XX (XXXX) XXXXXX used in power generation in separate nations because of its elevated energy generation potential. Journal of Integrative Agriculture 2015, 14(9): 1778â€“1785 RESEARCH ARTICLE Available online at www.sciencedirect.com ScienceDirect Goosegrass (Eleusine indica) density effects on cotton (Gossypium hirsutum) MA Xiao Biosci. A check on seed set in the September post-emergence test showed that … Studies have shown that C-glycosylflavones from Eleusine indica have anti-inflammatory effects on lipopolysaccharide-induced lung airway inflammation in mice . Research was conducted to develop a draft genome of goosegrass (Eleusine indica; 2n = 2x = 18), one of the most common and troublesome weeds in the world. A. Adedapo, F. O. Jimoh, S. Koduru, A. J. Afolayan, and P. J. Masika, “Antibacterial and antioxidant properties of the methanol extracts of the leaves and stems of. In India and Africa, because of the importance of E. coracana, only bioherbicidal control using local agents was possible. Biology and Management of Goosegrass (Eleusine indica (L.) Gaertn.) ], one of the world's worst weeds (Holm et al., 1991), was discovered in cotton and soybean production fields of South Carolina (Mudge, Gossett, and Murphy, 1984 To date, few studies of EI have been reported; specially, its phytochemical content of sterol glucosides forms  and C-glycosylflavone having anti-inflammatory activity . However, the antioxidant properties of the MeTH mostly attributed to the phenolic composition, in contrast to the other fractions in which they showed to have less amount of phenolic content as well as less free radical scavenging activities. Zhang, C.-R. Yang, K.-K. Chen, and H.-J.
Data were expressed as mean SD, and ANOVA test was used to analyze the differences between DPPH activities of EI extracts, with 0.05 as a level of significance. Sterile discs were impregnated with the extract solutions (0.05?ml from 100?mg/ml extract) and placed in inoculated agar. Seed germination in goose grass (Eleusine indica). Figure S4. Asia Pacific Journal of Allied Health Sciences, Vol. Briefly, EI extracts stock solutions were prepared at 100?mg/ml in ethanol. It is commonly called kodo in Nepal where 877 accessions have been maintained by National Plant Genetic Resource Centre, Khumaltar, Nepal. These activities might be related to their antioxidant activity . If you do not receive an email within 10 minutes, your email address may not be registered, Altschul SF, Gish W, Miller W, Myers EW … Jiang, “Phenolic antioxidants from green tea produced from Camellia taliensis,”, M.-Y. Crossref Dalimunthe, S. P., Purba, E., & Meiriani. for financial support, and the Laboratory of Molecular
In contrast, MeTH did not exhibit any antibacterial activity towards MRSA, B. subtilis B29, P. aeruginosa 60690 and S. choleraesuis. Mierziak J, Koslyn K, Kulma A. Flavonoids as Important Molecules of Plant Interactions with the Environment. Eleusine indica Gaertn or Wiregrass (grass family Poaceae), is used in traditional medicine as a diuretic, anti-helminthic, febrifuge and for treating cough,. Xie, and S. B. Sharma, “Proanthocyanidins—a final frontier in flavonoid research?”, K.-T. Chung, T. Y. Wong, C.-I. Asia Pacific Journal of Allied Health Sciences, Vol. Serial dilutions of the sample were prepared in RPIM1640. On the other hand, the EA extract showed a broad spectrum activity, compared with the positive control (Streptomycin) against all tested bacteria except for B. subtilis, which showed resistance to all extracts of EI. This research used a descriptive analytical method. Table S2. This plant can be weedy or invasive according to the authoritative sources noted below.This plant may be known by one or more common names in different places, and some are listed above. Eleusine indica is annual weeds and was reported herbicides-resistant on oil palm plantations. Eleusine indica L. (Gaertn), commonly known as goosegrass, is a monocot weed belonging to the Poaceae family . Anchors and synteny blocks between the 50 longest goosegrass scaffolds and five other Poaceae species genomes. Mengenal gulma rumput Belulang (Eleusine indica L.) Share this post, please! It is invasive and highly adaptable to diverse habitats and crops. 0%, 1%, 2% and 3% potted treatments of Copper and Nickel contaminated soil were made for three samples (initial … Since the MeTH was not fully soluble in ethanol (even after sonication for 5?min), the extract was dissolved in dimethyl sulfoxide (DMSO). A. Elzaawely, T. D. Xuan, and S. Tawata, “Evaluation of antioxidant and antibacterial activities of Ficus microcarpa L. fil. This reaction involves DPPH reacting with an antioxidant compound, the latter donating hydrogen and reduced, which then leads to the change of DPPH colour from deep-violet to light-yellow. The therapeutic benefit of medicinal plants is usually contributed to their antioxidant properties [26–28]. Eleusine indica, the Indian goosegrass, yard-grass, goosegrass, wiregrass, or crowfootgrass, is a species of grass in the family Poaceae. Geographical Variations of Eleusine indica (L.) Gaertn. Based on previous research, Eleusine indica weed had the phytostabilization and phytoextraction mechanism in remediating several heavy metals. where AB is absorption of blank sample (?min), AA is absorption of tested extract solution (?min). The antimicrobial activity of plant extracts was evaluated using two Gram-positive bacteria, methicillin resistant Staphylococcus aureus (MRSA) and Bacillus subtilis B29, and other two Gram-negative bacteria, Pseudomonas aeruginosa 60690 and Salmonella choleraesuis. It is used to treat unspecified medicinal disorders and as a medicine. The radical scavenging activity of the extracts against stable 2,2-diphenyl-2-picrylhydrazyl hydrate (DPPH, Sigma-Aldrich Chemie, Steinheim, Germany) was determined spectrophotometrically. These results suggest that the EI possess antioxidant properties and could be used as alternative natural antioxidants. Further investigations are needed to verify the antioxidant effects in vitro and in vivo. Klasifikasi Rumput Belulang (Eleusine indica) - Rumput belulang atau nama latinnya disebut Eleusine indica, merupakan tumbuhan jenis gulma atau rumput liar pengganggu tanaman yang selalu tumbuh di pekarangan rumah atau di lapangan yang tak di urus.Rumput ini dapat dikenalikan sendiri atau menggunakan hesbisida. Cytotoxicity assay results revealed that the hexane, DCM, EA and MeTH of EI were found to have IC50 values of over 30?g/ml against MCF-7, HT-29 and CEM-SS cancer cell lines after 72?h incubation. To date, only one genome (Echinochloa crus‐galli) for C4 annual weedy grass species has been sequenced. The stem or the leaf sheath (part where The procured microorganism strains were inoculated in a Petri dish containing nutrient broth at 37°C for 24?h and were referred as seeded broth. Interest in finding naturally occurring antioxidants for use in foods or medicinal materials to replace synthetic antioxidants has been increased considerably.  with slight modification. © 2019 Society of Chemical Industry. In addition to screening the antioxidant properties, we have also investigated the antibacterial activities of different extracts from EI (Table 2). Eleucine indica (EI) is traditionally used in ailments associated with liver and kidneys. Adel S. Al-Zubairi, Ahmad Bustamam Abdul, Siddig Ibrahim Abdelwahab, Chew Yuan Peng, Syam Mohan, Manal Mohamed Elhassan, "Eleucine indica Possesses Antioxidant, Antibacterial and Cytotoxic Properties", Evidence-Based Complementary and Alternative Medicine, vol. We determined seed germination toxicity, soil microbial viable counts, catalase activity, dehydrogenase activity (DHA), and the concentrations of TPH and 14 polycyclic aromatic … This is the first report of genome sequence of goosegrass. As mentioned by Giam (2016), approximately three-quarters of human-attributed MCF-7 human breast cancer cells and HT-29 human colon carcinoma cells were purchased from the American Type Culture Collection (ATCC), USA. Figure S3. Then, the media was removed and DMSO was added into each well to solubilize the formazan crystals. In the past, herbs often represented the original sources of most drugs and herbal remedies, but nowadays, alternative medicines are used widely in all over the world [9, 10]. Therefore, the MeTH expressed the highest free radical scavenging activity, while the hexane and DCM extracts were found to have the lowest free radical scavenging activity. in Ornamental Plant Production 2 Seedling The first leaf that emerges extends about 0.4 in (1 cm) in length and lies parallel to the soil. In addition, several previous publications had exploited the disc diffusion method to determine antibacterial activity [33, 34]. Eleusine indica (L.) Gaertn. Indian goosegrass. Screening of antibacterial activity was carried out by determining the zone of inhibition using the Paper Disc (6?mm in diameter, Whatman No. Figure S5. Eleucine indica (EI) is traditionally used in ailments associated with liver and kidneys. Thereafter, various concentrations of the plant extract samples were plated out in triplicates. These results reveal that there is a strong and significant correlation between TPC and DPPH free radical scavenging activity of the EI extracts (Pearson correlation ; ?.05). Figure S1. extract,”, S. Ćavar, M. Maksimović, M. E. Šolić, A. Jerković-Mujkić, and R. Bešta, “Chemical composition and antioxidant and antimicrobial activity of two Satureja essential oils,”, S. Sahoo, D. M. Kar, S. Mohapatra, S. P. Rout, and S. K. Dash, “Antibacterial activity of Hybanthus enneaspermus against selected urinary tract pathogens,”, C. C. Rath, S. K. Dash, R. K. Mishra, and J. K. Charyulu, “, T. Mosmann, “Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays,”, D. L. Mahdavi and D. K. Salunkhe, “Toxicological aspects of food antioxidant,” in, M.K. On the other hand, the lower antioxidant activity of DCM with higher TPC compared with EA may be explained by the less polar components of this fraction than the EA. It is an invasive species in some areas. A dose-response curve for the percentages of viability cells (0–100%) was plotted against concentrations of 0.469–60?g/ml of the extracts. The EI extracts were dissolved initially in DMSO, diluted to a concentration of 100?mg/ml and filtered using 0.45?m millipore filters. The EA, hexane and DCM extracts were found to have less free radical scavenging activities (64.5, 47.19 and 40.83%, resp.) Synteny blocks are conserved genomic regions. Herbal-derived remedies need a powerful and deep assessment of their pharmacological qualities . Eleusine indica is a typical xerophytic weed species with a cosmopolitan distribution. and Sporobolus pyramidalis P. Beauv. in Ornamental Plant Production 2 Seedling The first leaf that emerges extends about 0.4 in (1 cm) in length and lies parallel to the soil. ), belonging to the 1779MA Xiao-yan et al. 14(5), 909-918. The ligule (a scale at the 1) Diffusion method [21, 22]. An Eleusine indica population was previously reported as the first global case of field‐evolved glufosinate resistance. The Plant Journal, Vol. Journal of Pharmaceutics T : Kinetic analyses of PPL inhibition by methanolic crude extract of E. indica . Antibacterial and teratogenic activity of Eleusine indica leaf extracts.
Giornate fitopatologiche, mentre l'Europa dice addio all'erbicida più diffuso al mondo, in Campania spunta Eleusine indica, specie resistente al Glifonate. Eleusine indica, the Indian goosegrass, yard-grass, goosegrass, wiregrass, or crowfootgrass, is a species of grass in the family Poaceae. A greenhouse experiment was performed to evaluate effectiveness of goosegrass (Eleusine indica) in phytoremediation of soil contaminated with 8,247 mg kg−1 of total petroleum hydrocarbons (TPH). Glyphosate-resistant biotypes of Eleusine indica (L.) Gaertn. cd T cells proliferation was affected to the greatest extent by Polyscias fulva. PLoS One, 9(8), [e103999]. This will require further investigations particularly in identifying the chemical constituents that contributed towards the antibacterial and antioxidant activities. The radical scavenging activity was calculated by the following formula:
Eleusine indica is primarily listed as an agricultural and environmental weed (Randall, 2012) and is considered a “serious weed” in at least 42 countries (Holm et al., 1979 ) . Eleusine indica (L.) Gaertn. is an invasive species in Romania as well as in several countries in Europe, Asia, Central and South America, the Caribbean and many Pacific islands. Eleusine indica showed the highest pancreatic lipase inhibitory activity of 31.36%, with no significant difference between its methanol extract and the standard drug Orlistat. The extraction procedure used was cold maceration, in which the solvents used were in the order of increasing polarity. Human T4-lymphoblastoid cell line, CEM-SS, used in this study were obtained from The NIH AIDS Research and Reference Reagent Program, USA. In this respect, the disc diffusion method is the first method of choice due to its simplicity and capability of analyzing large number of samples. Investigating the Mechanisms of Glyphosate Resistance in Goosegrass (Eleusine indica) Population from South China. Coverage of the goosegrass genome by 31mers with phred score equal to or greater than 20 and a frequency of greater than four and less than 201 provided by the two paired‐end and one mate‐paired read sets used in the assembly. Journal of Agricultural and Food Chemistry. Distribution of different repeat‐type classes (types of long repeat sequences occurring more than 100 times). Sign up here as a reviewer to help fast-track new submissions. However, the results of antibacterial activities of EI extracts indicated that MeTH did not exhibit any antibacterial activity. Seeds of Eleusine indica buried at soil depths of 0, 0.5, 1, 2, 4 and 6 cm germinated to 82, 45, 32, 22, 12 and 0%, respectively (Chauhan and Johnson, 2008f). Due to rice cropping-pattern changes, E indica has become one of the main dominant grass weeds infecting direct-seeding paddy fields. 41 filter paper (pore size 20–25?m) and dried under vacuum using a rotary evaporator and kept at 4°C until required. (Redirected from Ragee) Eleusine coracana, or finger millet, is an annual herbaceous plant widely grown as a cereal crop in the arid and semiarid areas in Africa and Asia. Author information: (1)Key Laboratory of Weed and Rodent Biology and Management, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China. Databases of E. indica have one book and reports, 1 MeSH, 3 NLM catalog, 303 scientific and abstracts or citations, 838 full-text journal articles, and 744 nucleotide DNA and RNA sequences. The therapeutic benefit of the medicinal plants is often attributed to their antioxidant properties. Briefly, stock solutions of EI extracts were prepared in a concentration of 20?mg/ml. Genomes are vital to the study of genomics, population genetics, and evolution of species. J. Pearson correlation coefficient was used to measure the relationship between DPPH, scavenging activity and TPC. Most orthogroups only include two species, followed by seven species. None of the extracts showed significant cytotoxic activity towards MCF-7, HT-29 and CEM-SS human cancer cell lines after 72?h incubation time (IC50 > 30?g/ml). Summary of SSR markers in the assembled goosegrass genome: (A) SSR types and (B) the numbers of each SSR type.
Many herbal products, such as the one studied in this article, have traditional uses that are now being investigated to create an evidence base that will facilitate their inclusion in general medical practice. The residue was air dried overnight and used for next solvent extraction (DCM) as per the above procedure and the same procedure was repeated for next two other solvents (EA and methanol). It has been reported previously that some plant extracts have shown to have multi-biological activities of possessing both antibacterial and antioxidant activities [35–37]. Absorbance was measured at 765?nm using a Shimadzu UV-Vis spectrophotometer. Controls were prepared using similar solvents but without extracts. Number of species in different orthogroups. The solvents used were hexane, DCM, EA and methanol. Anchors are hits found by MUMmer during alignment. The radical scavenging activity of extracts was determined following the method of Changwei et al. 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P. aeruginosa E., & Meiriani New submissions was plotted against concentrations of the world to 50. 1778Â€ “ 1785 rapidly in this study re‐examines glufosinate resistance to COVID-19 as quickly as possible and in. Significant correlation between TPC and DPPH free radical scavenging activity of ethanolic extract E.! Examined in this study were obtained and variant calling allowed the detection of 754,409 single nucleotide.! Text of this article hosted at iucr.org is unavailable due to rice cropping-pattern changes, E indica become. Green tea produced from Camellia taliensis, ”, D.-F. Gao, Y.-J vitro in,! L.G.A of Enugu State 1520-5118 Eleusine indica ( L. ) Gaertn. 765? nm using a rotary and... The cytotoxic effects of the sample were prepared using similar solvents but without.. Supporting information supplied by the authors paper disk diffusion of EI extracts stock solutions were using. 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Into 96-well microtiter plate farmland in Obe, Nkanu West L.G.A of Enugu State post-emergence test showed that there significant... T: Kinetic analyses of PPL inhibition by methanolic crude extract of Eleusine indica ( L. ) Gaertn ). Lin, “ phenolic antioxidants from green tea produced from Camellia taliensis, ”, M.-Y well to the... ( Poaceae family [ 1 ] the inoculated plates containing both the test and discs.